The purpose of an experimental design is to improve the productivity of experimentation. It is an efficient procedure for planning\nexperiments, so the data obtained can be analyzed to yield a valid and objective conclusion. This approach has been used as an\nimportant tool in the optimization of different analytical approaches. A D-optimal experimental design was used here, for the\nfirst time, to optimize the experimental conditions for the detection of reactive oxygen species (ROS) produced by human blood\nfrom healthy donors, a biological matrix that better resembles the physiologic environment, following stimulation by a potent\ninflammatory mediator, phorbol-12-myristate-13-acetate (PMA). For that purpose, different fluorescent probes were used,\nas�����..
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